mouse igg anti oct4  (Santa Cruz Biotechnology)

Journal: Human Cell

Article Title: Novel CRISPR-Cas9 iPSC knockouts for PCCA and PCCB genes: advancing propionic acidemia research

doi: 10.1007/s13577-025-01193-z

Figure Lengend Snippet: Antibodies used in the study

Article Snippet: Pluripotency markers , Mouse IgG anti-Oct4 , 1:60 , Santa Cruz Cat# sc-5279, AB_628051.

Techniques: Immunocytochemistry, Cytometry, Western Blot

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: Characterization of iPSCs on 0 d and during neuronal differentiation on 7 d. A Scheme of modeling NSC differentiation: reference iPSC lines (WISCi004-B and WAi001-B), iPSC lines from one late-onset AD patient (MLUI007-J/H and MLUi008-B/F), and one iPSC line from one matched healthy control (MLUi009-A). B Phase contrast image for iPSCs and NSCs on 0 d and 7 d showing a homogenous differentiation into NSCs (scale bar left 300 μm; scale bar right 100 μm). White arrows indicate the rosette-like formation of NSCs representing a premature stage of neural rosettes. Data are shown for one iPSC line (clone) per donor. C Comparison of the three iPSC lines regarding their expression of pluripotency markers on 0 d and their expression of NSC markers on 7 d. We could prove the presence of pluripotency markers LIN28A, OCT4, NANOG, SOX2 in iPSCs and NSC markers SOX1, SOX2, NES, PAX6, MSI1 in NSCs. RT-PCR analysis by gel electrophoresis visualized as bar charts (one amplicon each from MLUi008-B, MLUi009-A, WISCi004-B was pooled for analysis; n = 3, mean ± SEM). D IF analysis on 0 d showed nuclear localization of OCT4 and cellular distribution of the epithelial marker ECAD in iPSCs (scale bar: 100 μm). E Telomere length measured by multiplex QRT-PCR in iPSCs on 0 d and in NSCs on 7 d; N = 3 differentiations, mean ± SEM

Article Snippet: Mouse IgG anti-human OCT4 (POU5F1) , IF: 1:100 , Santa Cruz , Sc-5279.

Techniques: Control, Comparison, Expressing, Reverse Transcription Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Amplification, Marker, Multiplex Assay, Quantitative RT-PCR

Journal: Molecular Neurobiology

Article Title: Neuronal Stem Cells from Late-Onset Alzheimer Patients Show Altered Regulation of Sirtuin 1 Depending on Apolipoprotein E Indicating Disturbed Stem Cell Plasticity

doi: 10.1007/s12035-023-03633-z

Figure Lengend Snippet: List of primers for transcript analysis

Article Snippet: Mouse IgG anti-human OCT4 (POU5F1) , IF: 1:100 , Santa Cruz , Sc-5279.

Techniques:

Journal: Biotechnology progress

Article Title: Induced pluripotent stem cells can utilize lactate as a metabolic substrate to support proliferation.

doi: 10.1002/btpr.3090

Figure Lengend Snippet: Figure 8. Comparison of pluripotency gene and protein expression for K3 iPSCs after one and five passages under different initial glucose and lactate concentrations. A) Normalized gene expression after 1-passage for Oct4, Sox2, and Nanog. B) Normalized protein expression after 1-passage for Oct4. C) Normalized gene expression after 5-passages for Oct4, Sox2, and Nanog. D) Normalized protein expression after 5-passages for Oct4. Control media – blue; Low Glucose media – light blue; High Lactate media – red; and Low Glucose + High Lactate – light red. The asterisk (*) indicates that the normalized gene or protein expressions were significantly different from the control (p ≤ 0.05). Error bars represent standard error.

Article Snippet: The secondary antibody incubations were conducted for 1-h at room temperature at a 1:1000 dilution in TBST using a goat anti-rabbit IgG HRP- linked antibody for β-actin and a goat anti-mouse IgG HRP-linked antibody for Oct4 (Bio-Rad Laboratories, Inc., Hercules, CA).

Techniques: Comparison, Expressing, Gene Expression, Control